This course is INACTIVE
School
Science, Technology, Engineering and Math
Division
Science - DNU
Department
Biology
Academic Level
Undergraduate
Course Subject
Biology
Course Number
262
Course Title
Proteins
Credit Hours
5.00
Instructor Contact Hours Per Semester
107.00 (for 15-week classes)
Student Contact Hours Per Semester
107.00 (for 15-week classes)
Grading Method
A-E
Pre-requisites
CHEM-141 and BIO-152 with grades of C or better
Catalog Course Description
Focuses on the structure and function of proteins along with chemical and physical techniques commonly used when working with these molecules. Lectures and laboratories emphasize the principles and applications of modern biochemical analyses used in biotechnology and related proteomic fields. This course is designed to satisfy the requirements of the Biotechnology Program; a grade of C or better is required to satisfy the requirements of the Biotechnology Program. Seven hours of integrated lecture and laboratory per week.
Goals, Topics, and Objectives
Core Course Topics
- Amino Acids
- Protein Structure and Function
- Protein Isolation, Purification, and Quantitation
- Tissue Culture
- Chromatography: size exclusion; ion-exchange; affinity
- Electrophoresis: SDS-Page; Western Blot
- Spectroscopy: UV/VIS; Fluorescence
- Enzymes: Enzyme activity analysis
- Scientific method and experimental design
- Biotechnology Laboratory Skills, including following written protocols, SOPs, GLPs; specimen handling and storage; laboratory recordkeeping; laboratory safety; communication; professional behavior; teamwork
Core Course Learning Objectives (Separated)
- Organize the twenty common amino acids in accordance with the chemical properties of their side chains; explain how amino acids are combined to form proteins and describe the levels of protein structure.
- Identify forces that contribute to protein stabilization and factors that disrupt them.
- Calculate the isoelectric point and the net charge of a peptide at a given pH.
- Identify factors that influence enzyme activity and discuss mechanisms of enzyme activity.
- Demonstrate the ability to predict reactions between proteins by an understanding of functional group reactivity.
- Explain standard techniques and protocols used to: isolate, purify, and quantitate proteins, and evaluate a protein's function.
- Describe the three-dimensional structures of proteins; explain how the three-dimensional structures of proteins relate to function.
- Determine the appropriate application of standard protein-related techniques, and explain how to achieve particular outcomes using appropriate protein-related techniques.
- Demonstrate proficiency with the use of the following equipment: centrifuge, laminar flow hood, incubator, balance, volumetric glassware and pipettes, spectrophotometer, fluorometer, plate reader, electrophoresis apparatus, chromatography apparatus, imaging system, sonicator.
- Prepare, dispense, and appropriately label reagents; maintain reagent integrity.
- Perform basic laboratory calculations.
- Demonstrate proficiency with the following techniques: protein isolation from multiple sources, protein quantitation, mammalian tissue culture, centrifugation, chromatography (ion-exchange, size exclusion, affinity), enzyme activity analysis, fluorescence spectroscopy, UV/VIS spectroscopy, and electrophoresis.
- Apply the scientific method; design and perform experiments according to accepted methods.
- Demonstrate laboratory safety skills, including identifying safety hazards, employing preventive safety measures, complying with safety and emergency procedures, and demonstrating appropriate care and use of equipment and facilities to ensure safety; demonstrate appropriate handling and disposal of chemical and biological materials in keeping with SDS and worker protection standards (WPS).
- Demonstrate scientific accountability and ethical behavior.
- Recognize and apply appropriate documentation requirements.
- Demonstrate effective teamwork skills and coordinate tasks with lab partners.
- Display professional behavior, courtesy, and attitude.
- Troubleshoot laboratory problems and apply decision-making and problem-solving techniques in the laboratory.
- Follow standard operating procedures (SOPs) and good laboratory practices (GLPs).
- Demonstrate the use of critical thinking skills in the analysis and interpretation of experimental data.
- Communicate in a professional manner.
- Recognize and use a technical vocabulary.
- Integrate relevant information from diverse sources.
General Information
This course was part of the Biotechnology program and certificate. It is no longer needed and the teach out period will expire at the start of the summer semester.
Assessment and Requirements
Assessment of Academic Achievement
Student achievement is assessed through quizzes, assignments, laboratory reports, skills demonstrations, presentations and exams.
Outcomes
General Education Categories
- Natural Sciences
Institutional Outcomes
- Scientific Reasoning
MTA Categories
- Category 6: Natural Sciences (Lecture and Lab)
Satisfies Wellness Requirement
No
Approval Dates
Effective Term
Summer 2023